G 418 disulfate salt G 418 二硫酸盐
CAS 108321-42-2 MFCD00058314
化学结构图
SMILES: CC(O)[C@@H]1O[C@@H](O[C@@H]2[C@@H](O)[C@H](O[C@H]3OC[C@](C)(O)[C@H](NC)[C@H]3O)[C@@H](N)C[C@H]2N)[C@@H](N)[C@H](O)[C@H]1O.OS(O)(=O)=O.OS(O)(=O)=O |&1:3,5,7,8,10,12,15,18,21,23,26,28,30,32|
化学属性
| Mol. Formula | C20H40N4O10.2H2SO4 |
|---|---|
| Mol. Weight | 693 |
| Melting Point | 138-144°C |
别名和识别编码
| Chemical Name | G 418 disulfate salt |
|---|---|
| Synonym | Antibiotic G418 G 418 disulfate salt solution G 418 二硫酸盐 |
| MDL Number | MFCD00058314 |
| PubChem Substance ID | 125307225 |
| CAS Number | 108321-42-2 |
| Chemical Name Translation | G 418 二硫酸盐 |
| LabNetwork Molecule ID | LN00194378 |
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分类
- {SNA} A - K, Aminogl
- {SNA} A - K, Aminoglycosides, Antibacterial, Antibiotic Solutions, Antibiotics, Antibiotics A to Z, Antibiotics G-M, Antibiotics by Application, Antineoplastic and Immunosuppressive Antibiotics, Biochemicals and Reagents, Cell Culture, Chemical Structure Class, Core Bioreagents, Genetic Marker Selection, Interferes with Protein Synthesis, Life Science Reagents for Cell Culture, Mechanism of Action, Reagents and Supplements, Research Essentials, Spectrum of Activity
- {SNA} A - K,
- {SNA} A - K, Aminoglycosides, Antibacterial, Antibiotic powders,
产品应用
- 靠干扰核糖体功能阻断多肽合成。不是常用的标准抗生素，常用于选择新霉素抗药基因转染的原核和真核细胞。
- 在基因转移、基因敲除、抗性筛选以及转基因动物等方面得以广泛应用。
相关文献及参考
- 1. G. H. Wagman, R. T. Testa, et al., Antibiotic G-418, a New Micromonospora-Produced Aminoglycoside with Activity Against Protozoa and Helminths: Fermentation, Isolation, and Preliminary Characterization Antimicrob. Agents Chemother. 6 , 144-149, (1974)
- 2. Outi Sareila, Mari Hämäläinen, et al., Orazipone Inhibits Activation of Inflammatory Transcription Factors Nuclear Factor-B and Signal Transducer and Activator of Transcription 1 and Decreases Inducible Nitric-Oxide Synthase Expression and Nitric Oxide Production in Response to Inflammatory Stimuli J. Pharmacol. Exp. Ther. 324 , 858-866, (2008)
- Canaani, D., and Berg, P., Regulated expression of human interferon β1 gene after transduction in to cultured mouse and rabbit cells. Proc. Natl. Acad. Sci. U. S. A. 79 , 5166-5170, (1982) 摘要
- Southern, P., and Berg, P., Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Biol. 1 , 327-341, (1982) 摘要
- Halaban, R., and Alfano, F., Selective elimination of fibroblasts from cultures of normal human melanocytes. In Vitro Cell. Dev. Biol. 20 , 447-450, (1984) 摘要
- Ursic, D., et al., A new antibiotic with known resistance factors, G418, inhibits plant cells. Biochem. Biophys. Res. Commun. 101 , 1031-1037, (1981) 摘要
- Bar-Nun, S., et al., G-418, an elongation inhibitor of 80 S ribosomes. Biochim. Biophys. Acta 741 , 123-127, (1983) 摘要
- Kingston, R, Ausubel, F. et al., Stable transfer of genes into mammalian cells. Short Protocols in Molecular Biology 2nd ed., New York , (1992), 9.14
安全信息
- S24/25 Avoid contact with skin and eyes 避免皮肤和眼睛接触；
GHS Symbol
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray. 避免吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
- P262 Do not get in eyes, on skin, or on clothing. 不要接触眼睛，皮肤或衣服。
- P280 Wear protective gloves/protective clothing/eye protection/face protection. 戴防护手套/防护服/眼睛的保护物/面部保护物。
- P342+P311
- H317 May cause an allergic skin reaction 可能导致皮肤过敏
- H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled 吸入可能导致过敏或哮喘症状或呼吸困难
- R42/43
其他信息
- 溶于水、甲醇。 G418是一种氨基糖苷类抗生素 ,这种氨基糖类抗生素的结构和新霉素、庆大霉素、卡那霉素相似，在分子遗传试验中,是稳定转染最常用的抗性筛选试剂。它通过抑制转座子Tn601，Tn5的基因,干扰核糖体功能而阻断蛋白质合成,对原核和真核等细胞产生毒素 ,包括细菌、酵母、植物和哺乳动物细胞 ,也包括原生动物和蠕虫。当neo基因被整合进真核细胞DNA后 ,则能启动neo基因编码的序列转录为mRNA ,从而获得抗性产物氨基糖苷磷酸转移酶的高效表达 ,使细胞获得抗性而能在含有 G418的选择性培养基中生长
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