Fluorescamine 荧光胺

CAS 38183-12-9 MFCD00005928

化学结构图

38183-12-9
SMILES: O=C1C(=COC21OC(=O)C1C=CC=CC2=1)C1C=CC=CC=1

化学属性

Mol. FormulaC17H10O4
Mol. Weight278
Melting Point150-160
TSCANo
Density1.422
Flash Point-17 °C

别名和识别编码

Chemical NameFluorescamine
Synonym 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3'-dione 4-Phenylspiro[furan-2(3H),1'-phthalan]-3,3'-dione Fluram 荧光胺
MDL NumberMFCD00005928
PubChem Substance ID87569975
Beilstein Registry Number921143
CAS Number38183-12-9
EC Number253-814-5
Reaxys-RN921143
Merck Number4158
Chemical Name Translation荧光胺
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分类

  • {SNA} Alphabetical, Amino Acid Analysis, Amino Acids, Carbohydrate Labels, Carbohydrate Labels for Chromatography, Chemical Biology, Chemical Synthesis, Derivatization Reagents, Derivatization Reagents HPLC, Detection, Detection Products for Amino Acid Analysis, Fluorescamine, Fluorescence, Fluorescent Labels, Fluorescent Stains, Functional Group Reactive Labels, Functional Group Reactive Labels for Chromatography, Functional Group Reactive Labels for Electrophoresis, Functional Group Reactive Labels for Flow Cytometry, HPLC Derivatization Reagents, by Application, Ketones, Peptide Analysis and Characterization, Peptide Chemistry,
  • {SN

产品应用

  • 用于高效液相色谱标记，也能在温和的条件下和氨基酸和蛋白质的伯氨基形成稳定、高荧光、低背景的复合物， 用于荧光检测氨基酸、蛋白质和蛋白水解酶。也用于蛋白测序分析中。

相关文献及参考

  • 3. Sogowa, K., and Takahaski, K., Use of fluorescamine-labeled casein as a substrate for assay of proteinases. J. Biochem. Tokyo 83 , 1783, (1978) 摘要
  • Stein, S., et al., Amino acid analysis with fluorescamine at the picomole level. Arch. Biochem. Biophys. 155 , 202, (1973) 摘要
  • Bhown, A.S., et al. Anal. Biochem. 112 , 158, (1981)
  • Merck: 14,4158

安全信息

Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany3
Signal word Warning
GHS Symbol
Safety Statements
  • S22 Do not breathe dust 不要吸入粉尘；
  • S24/25 Avoid contact with skin and eyes 避免皮肤和眼睛接触；
  • S26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice 眼睛接触后，立即用大量水冲洗并征求医生意见；
  • S36/37/39 Wear suitable protective clothing, gloves and eye/face protection 穿戴适当的防护服、手套和眼睛/面保护；
Risk Statements
  • R36/37/38 Irritating to eyes, respiratory system and skin 对眼睛、呼吸系统和皮肤有刺激性
Precautionary statements
  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray. 避免吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
  • P305+P351+P338
Hazard Codes F,Xi
Storage condition 室温保存

其他信息

  • 本身与水解产物不显荧光。但与含有伯氨基的化合物(包括蛋白质、肽、氨基酸及其他各种多肽)分别生成类似的、具有强烈荧光性的化合物。生成物的最大激发波长为390nm，最大发射波长为475nm。

系列性分类

  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Fluorescent Probes, Labels, Particles and Stains - Biochemicals and Reagents
  4. Detection - Fluorescent Probes, Labels, Particles and Stains
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Luminescent Compounds/Detection - Biochemicals and Reagents
  4. Wavelength Index - Luminescent Compounds/Detection
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Fluorescent Probes, Labels, Particles and Stains - Biochemicals and Reagents
  4. Fluorescent Labels - Fluorescent Probes, Labels, Particles and Stains
  5. Other Fluorescent Labels - Fluorescent Labels
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Fluorescent Probes, Labels, Particles and Stains - Biochemicals and Reagents
  4. Functional Group Reactive Labels - Fluorescent Probes, Labels, Particles and Stains
  5. Functional Group Reactive Labels for Flow Cytometry - Functional Group Reactive Labels
  1. Analytical & Chromatography 分析化学与色谱
  2. Analytical Reagents
  3. Derivatization Reagents 衍生分析试剂
  4. Derivatization Reagents HPLC - Derivatization Reagents
  5. Fluorescence - Derivatization Reagents HPLC
  6. Ketones - Fluorescence
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Peptides - Biochemicals and Reagents
  4. Peptide Analysis and Characterization - Peptides
  5. Amino Acid Analysis - Peptide Analysis and Characterization
  6. Amino Acid Analysis Reagents - Amino Acid Analysis
  1. Life Science 生命科学
  2. Proteomics 蛋白质组学
  3. Protein Electrophoresis - Proteomics
  4. Protein Stains and Staining Reagents - Protein Electrophoresis
  5. Other Stains for Electrophoresis - Protein Stains and Staining Reagents
  1. Chemical Synthesis 化学合成
  2. Building Blocks 合成砌块
  3. Organic Building Blocks 有机砌块
  4. Carbonyl Compounds 羰基化合物
  5. Aromatic acids
  1. Analytical & Chromatography 分析化学与色谱
  2. Labeling Reagents for HPLC
  1. Functional Group 官能团索引
  2. enol ether
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Luminescent Compounds/Detection
  4. Chemiluminescent compounds
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Fluorescent Probes, Labels, Particles and Stains
  4. Fluorescent Labels
  5. Fluorescein and Derivatives
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Fluorescent Probes, Labels, Particles and Stains
  4. Functional Group Reactive Labels
  5. Functional Group Reactive Labels for Electrophoresis
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Proteomics
  4. Protein Quantitation
  5. General Protein Quantitation
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Luminescent Compounds/Detection
  4. Fluorescent Probes, Labels, Particles and Stains
  5. Fluorescent Labels
  6. Fluorescein and Derivatives
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Luminescent Compounds/Detection
  4. Fluorescent Probes, Labels, Particles and Stains
  5. Functional Group Reactive Labels
  6. Functional Group Reactive Labels for Electrophoresis
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Molecular Biology
  4. Protein Electrophoresis
  5. Protein Stains and Staining Reagents
  6. Fluorescent Protein Electrophoresis Stains
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Proteomics
  4. Protein Electrophoresis
  5. Protein Stains and Staining Reagents
  6. Fluorescent Protein Electrophoresis Stains
  1. Life Science 生命科学
  2. Biochemicals and Reagents
  3. Plant Biotechnology
  4. Plant Proteomics
  5. Protein Electrophoresis
  6. Protein Stains and Staining Reagents
  7. Fixing Reagents

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