Deoxyribonuclease I From Bovine Pancreas 脱氧核糖核酸酶I来源于牛胰腺

CAS 9003-98-9 MFCD00130918

化学结构图

9003-98-9
SMILES:

化学属性

Mol. Formula-
Density100 g/mL at 20 °C
Stability易潮湿
Mol. Weight-

别名和识别编码

Synonym Alkaline DNase; Alkaline deoxyribonuclease; DNA depolymerase; DNA endonuclease; DNA nuclease; DNAase; DNase; Deoxyribonuclease A; Deoxyribonuclease I; Deoxyribonucleic phosphatase; Desoxyribonuclease; Dornase; Pancreatic deoxyribonuclease; Pancreatic dornase Alkaline deoxyribonuclease DNA depolymerase DNAse I 脱氧核糖核酸 5′-寡核苷酸-水解酶 DNA酶I DNase I 脱氧核糖核酸 5′-寡核苷酸-水解酶 Deoxyribonuclease Deoxyribonuclease (pancreatic) Deoxyribonuclease A Deoxyribonuclease I Deoxyribonuclease I from Bovine Pancreas {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
MDL NumberMFCD00130918
CAS Number9003-98-9
EC Number232-667-0
Merck Number2906
Chemical NameDeoxyribonuclease I From Bovine Pancreas
Chemical Name Translation脱氧核糖核酸酶I来源于牛胰腺
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分类

  • {SNA} 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Apoptosis and Cell Cycle, Application Index, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Enzymes for Diagnostic Kit Manufacturing, Molecular Diagnostics, Other Apoptosis Related Reagents, Protein & Nucleic Acid Isolation, 细胞信号转

产品应用

  • 用于从蛋白质样品中除去 DNA。
  • Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.

相关文献及参考

  • Merck: 14,2906

安全信息

WGK Germany3
RTECSRF0750000
Storage condition 储存温度2-8℃ 0-10°C 储存温度 -20°C 0-10 储存温度-20°C 储存温度-20°
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
GHS Symbol
Safety Statements
  • S24/25 Avoid contact with skin and eyes 避免皮肤和眼睛接触；
Precautionary statements
  • P262 Do not get in eyes, on skin, or on clothing. 不要接触眼睛，皮肤或衣服。
TYPE OF TEST            : Cytogenetic analysis
TEST SYSTEM             : Insect - Drosophila melanogaster Cells - not
                          otherwise specified
DOSE/DURATION           : 1 gm/L
REFERENCE :
   NULSAK Nucleus (Calcutta).  (Dr. A.K. Sharma, Centre of Advanced Studies in
   Cell and Chromosome Research, Calcutta, 35 Baliygunge Circular Rd., Calcutta
   700 019, India)  V.1-    1958-  Volume(issue)/page/year: 2,85,1959

TYPE OF TEST            : Cytogenetic analysis
TEST SYSTEM             : Insect - grasshopper Cells - not otherwise specified
DOSE/DURATION           : 1 gm/L
REFERENCE :
   NULSAK Nucleus (Calcutta).  (Dr. A.K. Sharma, Centre of Advanced Studies in
   Cell and Chromosome Research, Calcutta, 35 Baliygunge Circular Rd., Calcutta
   700 019, India)  V.1-    1958-  Volume(issue)/page/year: 2,85,1959

TYPE OF TEST            : Sister chromatid exchange
TEST SYSTEM             : Rodent - hamster Ovary
DOSE/DURATION           : 2500 units
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 307,315,1994

TYPE OF TEST            : DNA damage
TEST SYSTEM             : Rodent - hamster Fibroblast
DOSE/DURATION           : 5000 ppm
REFERENCE :
   SFCRAO Collection of Papers Presented at the Annual Symposium on Fundamental
   Cancer Research. (Baltimore, MD)  V.1-29, 1946-76.  For publisher
   information, see AFCPDR.  Volume(issue)/page/year: 23,346,1970

其他信息

  • 牛胰腺脱氧核糖核酸酶（又称DNA酶）是一种核酸内切酶，它作用于磷酸二脂键，特别针对毗邻嘧啶核苷的键，从而产生3' 末端带自由羟基和5' 末端带磷酸盐的多聚核苷酸。酶作用的最佳pH值为7.8。DNA酶能被二价金属活化，被鳌合物如EDTA和十二烷基硫酸钠抑制。浓度为5 mM的钙离子可作为稳定剂，以保护DNA酶不被水解酶分解。
  • Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.

系列性分类

  1. Research Essentials 基础科研
  2. Core Bioreagents - Research Essentials
  3. Enzymes - Core Bioreagents
  1. Life Science 生命科学
  2. Metabolomics 代谢组学
  3. General Metabolic Enzymes - Metabolomics
  4. Nucleotide Metabolism - General Metabolic Enzymes
  1. Life Science 生命科学
  2. Molecular Biology 分子生物学
  3. Molecular Biology Enzymes - Molecular Biology
  4. Modifying Enzymes - Molecular Biology Enzymes
  1. Life Science 生命科学
  2. Molecular Biology 分子生物学
  3. Molecular Biology Enzymes - Molecular Biology
  4. Modifying Enzymes - Molecular Biology Enzymes
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Enzymes, Inhibitors, and Substrates - Biochemicals and Reagents
  4. Application Index - Enzymes, Inhibitors, and Substrates
  5. Enzymes for Diagnostic Kit Manufacturing - Application Index
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Enzymes, Inhibitors, and Substrates - Biochemicals and Reagents
  4. Application Index - Enzymes, Inhibitors, and Substrates
  5. Protein & Nucleic Acid Isolation - Application Index
  1. Life Science 生命科学
  2. Proteomics 蛋白质组学
  3. Native Protein Sample Preparation - Proteomics
  4. Cell Lysis and Protein Extraction Reagents - Native Protein Sample Preparation
  5. Protein Sample Clarification - Cell Lysis and Protein Extraction Reagents
  1. Life Science 生命科学
  2. Cell Biology 细胞生物学
  3. Cell Signaling and Neuroscience - Cell Biology
  4. Apoptosis and Cell Cycle - Cell Signaling and Neuroscience
  5. Other Apoptosis Related Reagents - Apoptosis and Cell Cycle
  6. Enzymes - Other Apoptosis Related Reagents
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Enzymes, Inhibitors, and Substrates - Biochemicals and Reagents
  4. Enzyme Class Index - Enzymes, Inhibitors, and Substrates
  5. 3.x.x.x Hydrolases - Enzyme Class Index
  6. 3.1.x.x Acting on esters - 3.x.x.x Hydrolases
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Enzymes, Inhibitors, and Substrates - Biochemicals and Reagents
  4. Application Index - Enzymes, Inhibitors, and Substrates
  5. Molecular Biology Tested - Application Index
  6. Protein and Nucleic Acid Isolation - Molecular Biology Tested
  1. Life Science 生命科学
  2. Biochemicals and Reagents 生化试剂
  3. Enzymes, Inhibitors, and Substrates - Biochemicals and Reagents
  4. Analytical Enzymes - Enzymes, Inhibitors, and Substrates
  5. Recombinant Alternatives to Animal Source Proteins - Analytical Enzymes
  6. Recombinant Alternatives to Bovine Source Proteins - Recombinant Alternatives to Animal Source Proteins
  1. Life Science 生命科学
  2. Biochemistry / Glyco-Chemistry
  3. Enzyme

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