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• 试剂商城
• 直接询价

分类

• {SNA} 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology, Enzyme Class Index, Enzymes, In
• {SA} 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index, En

产品应用

• Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

相关文献及参考

• 1. E. Volkin & W.E. Cohn, On the structure of ribonucleic acids. II. The products of ribonuclease action J. Biol. Chem. 205 , 767-782, (1953)
• Agarraberes, F.A., and Dice, J.F., A molecular chaperone complex at the lysosomal membrane in required for protein translocation. J. Cell Sci. 114 , 2491, (2001)
• Kashio, Y., et al., Galectin-9 induces apoptosis through the calcium-calpain-caspase-1 pathway. J. Immunol. 170 , 3631, (2003)
• Merck 14 ,8202

安全信息

GHS Symbol

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intramuscular
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 310 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   54IIAL "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie.
   Nucleases: Biological Function and Practical Applications," Berdyshev, G.D.,
   et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985
   Volume(issue)/page/year: -,56,1985

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 392 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   54IIAL "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie.
   Nucleases: Biological Function and Practical Applications," Berdyshev, G.D.,
   et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985
   Volume(issue)/page/year: -,56,1985

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 290 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   54IIAL "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie.
   Nucleases: Biological Function and Practical Applications," Berdyshev, G.D.,
   et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985
   Volume(issue)/page/year: -,56,1985

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intraperitoneal
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 250 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   54IIAL "Nukleazy: Biologicheskaya Rol i Prak

TYPE OF TEST            : Mutation test systems - not otherwise specified
TEST SYSTEM             : Microorganism - not otherwise specified
DOSE/DURATION           : 40 mg/L
REFERENCE :
   AMICCW Archives of Microbiology.  (Springer-Verlag New York, Inc., Service
   Center, 44 Hartz Way, Secacus, NJ 07094)  V.95-    1974-
   Volume(issue)/page/year: 122,161,1979

其他信息

• 核糖核酸酶能专一性地作用于嘧啶碱基，分解RNA从而得到含有3'-UMP，3'-CMP，及其低聚核苷酸。核糖核酸酶是由单链多肽链组成，为分子量约1.4万的球状蛋白质，虽对于稀酸、热、蛋白质分解酶极为稳定，但在碱中不稳定。
• Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A. Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.
• Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5.