• 原料直供
• 试剂商城
• 直接询价

分类

• {SNA} 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Dissociation, Cell Dissociation and Cell Lysis, Chymotrypsin, al
• {SNA} 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Chymotrypsin, alpha-, Enzymatic and Chemical Protein Cleavage, Enzyme Class Index, Proteases for Cleavage of Proteins, Proteolytic Enzymes, Proteolytic Enzymes and Substrates, Selective Proteolytic Enzymes, 分子生物学, 生化试剂, 蛋白质组学, 质谱, 酶、抑制剂和底物
• {SNA} 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Biochemicals and Reagents, Enzyme Class Index, Enzy

产品应用

• 一种丝氨酸蛋白酶，水解肽键的羧基端带有芳香或长疏水链(Tyr, Trp, Phe, Met)的肽键。
• 备注：用于没有外源胰蛋白酶活性存在条件下的蛋白质水解。

相关文献及参考

• D'Arienzo, R., et al., Modulation of the immune response by probiotic strains in a mouse model of gluten sensitivity. Cytokine 48 , 254-9, (2009) 摘要
• D'Arienzo, R., et al., Adjuvant effect of Lactobacillus casei in a mouse model of gluten sensitivity. Immunol. Lett. 119 , 78-83, (2008) 摘要
• Ehren, J., et al., A food-grade enzyme preparation with modest gluten detoxification properties. PLoS Biol. 4 , e6313, (2009) 摘要
• Merck 14 ,2260
• Matthew, S., et al., Largamides A-C, tiglic acid-containing cyclodepsipeptides with elastase-inhibitory activity from the marine cyanobacterium Lyngbya confervoides. Planta Med. 75 , 528-33, (2009) 摘要
• Cleemann, F., and Karuso, P., Fluorescence anisotropy assay for the traceless kinetic analysis of protein digestion. Anal. Biochem. 80 , 4170-4, (2008) 摘要
• Ziemert, N., et al., Exploiting The Natural Diversity Of Microviridin Gene Clusters For Discovery Of Novel Tricyclic Depsipeptides. Appl. Environ. Microbiol. 76 , 3568-74, (2010) 摘要
• Merck: 14,2260
• Karuso, P., et al., Real-time fluorescence monitoring of tryptic digestion in proteomics. J. Proteome Res. 7 , 361-6, (2008) 摘要

安全信息

GHS Symbol

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intramuscular
SPECIES OBSERVED        : Rodent - rat
DOSE/DURATION           : 116 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not r

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intramuscular
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 104 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported oth

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Subcutaneous
SPECIES OBSERVED        : Rodent - mouse
DOSE/DURATION           : 185 mg/kg
TOXIC EFFECTS :
   Details of toxic effects not reported other than lethal dose value
REFERENCE :
   NIIRDN Drugs in Japan (Ethical Drugs).  (Yakugyo Jiho Co., Ltd., Tokyo,
   Japan)  Volume(issue)/page/year: 6,202,1982

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPO

TYPE OF TEST            : LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - guinea pig
DOSE/DURATION           : 50000 units/kg
TOXIC EFFECTS :
   Sense Organs and Special Senses (Olfaction) - effect, not otherwise
   specified
   Cardiac - other changes
   Blood - hemorrhage
REFERENCE :
   AIPTAK Archives Internationales de Pharmacodynamie et de Therapie. (Heymans
   Institute of Pharmacology, De Pintelaan 185, B-9000 Ghent, Belgium)  V.4-   
   1898-  Volume(issue)/page/year: 106,164,1956

TYPE OF TEST            : LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE       : Intravenous
SPECIES OBSERVED        : Rodent - rabbit
DOSE/DURATION           : 24000 units/kg
TOXIC EFFECTS :
   Gastrointestinal - other changes
   Blood - hemorrhage
   Skin and Appendages - dermatitis, other (after systemic exposure)
REFERENCE :
   AIPTAK Archives Internationales de Pharm

其他信息

• 糜蛋白酶又称胰凝乳蛋白酶，是一种蛋白水解酶，它能优先水解L-酪氨酸和L-苯丙氨酸的羧基所形成的肽键。酶作用最优化的PH值为8.0～9.0。糜蛋白酶活性能被重金属和天然胰蛋白酶抑制剂不同程度地抑制。白色或类白色结晶状。分子量约25 kDa。 Enzyme Commission (EC) Number: 3.4.21.1
• Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues.